BioEdit is a biological sequence alignment editor written for Windows 95/98/NT/2000/XP/78/10.  An intuitive multiple document interface with convenient features  makes alignment and manipulation  of sequences relatively easy on your desktop computer.    Several sequence manipulation and analysis options and links to external analysis programs facilitate a working environment which allows you to view and manipulate sequences with simple point-and-click operations.
  BioEdit's features include:                   

  • Several modes of hand alignment
  • Automated ClustalW alignment
  • Automated Blast searches (local and WWW)
  • Plasmid drawing and annotation
  • Accessory application configuration
  • Restriction mapping
  • RNA comparative analysis tools
  • Graphical matrix data viewing tools
  • Shaded alignment figures
  • Translation-based nucleic acid alignment
  • ABI trace viewing, editing and printing
  • Customizable ... other features

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    In some computers running Windows 10, the BioEdit Setup.exe file (created with an old version of InstallShield Express) may generate a virus alert in Windows Defender, even though Windows Defender will not detect a virus if the file is scanned directly. If this happens, testing suggests that the installer executing in memory will still complete and BioEdit will install OK, even though Windows Defender will automatically remove Setup.exe from the disk.

    If this is an issue or is concerning to you, the full package of files required for running BioEdit is packaged in without an installer program. The full contents of can simply be unzipped to a single folder and BioEdit.exe can be run from that folder. Before unzipping or running Bioedit.exe, however, it is advisable to do a virus scan with whatever scanner you trust. Also, checking the MD5 or SHA1 checksum is not a bad idea for the security conscious.
    SHA1 checksum for 688d6607f0ace12f93a92019788f07231e2aef1c
    MD5 checksum: for a2abd9b2373905dd4bd1fc610f62e3ee
    SHA1 checksum for 464122273e30a9c2e418ae8b109d1db9933f4ff4
    MD5 checksum: for 434aae3df9243a74700536b6a23744dd

    The checksum of a file can be generated in a Windows command shell with the CertUtil command:
    CertUtil -hashfile MD5 (for MD5), or
    CertUtil -hashfile (for SHA1)

    Note: BioEdit is no longer being regularly maintained, and the documentation is out of date and no longer maintained.  BioEdit is free for use by any and all interested parties, but is supplied as is. Users must agree with the terms of the license.

    Note also that the web site that BioEdit was hosted on for many years ( no loger exists (nor does the RNase P database), as Dr. James Brown has retired, the Microbiology department at NCSU has been reorganized, and the server no longer exists. For this reason, the ancient "BioEdit" web site has been minimally migrated to a Github Pages site to allow people to continue to download the program if they still find it useful.


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    Copyright © 1999, Inc.
       Software Informer
     Current version
     Last updated 5/10/2021
     Tom Hall